TheCBP2 gene fromSaccharomyces douglasii is a functional homologue of theSaccharomyces cerevisiae gene and is essential for respiratory growth in the presence of a wild-type (intron-containing) mitochondrial genome |
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| Authors: | G-Y Li G-L Tian P P Slonimski and C J Herbert |
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| Institution: | (1) Centre de Génétique Moléculaire, Laboratoire propre du CNRS, associé à l'Université Pierre et Marie Curie, F-91198 Gif-sur-Yvette, France;(2) Present address: V. A. Medical Center (151B), 4500 South Lancaster Rd., Dallas Texas, USA;(3) Present address: Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511 Singapore, Republic of Singapore |
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| Abstract: | InSaccharomyces cerevisiae the only known role of theCBP2 gene is the excision of the fifth intron of the mitochondrialcyt b gene (bI5). We have cloned theCBP2 gene fromSaccharomyces douglasii (a close relative ofS. cerevisiae). A comparison of theS. douglasii andS. cerevisiae sequences shows that there are 14% nucleotide substitutions in the coding region, with transitions being three times more frequent than transversions. At the protein level sequence identity is 87%. We have demonstrated that theS. douglasii CBP2 gene is essential for respiratory growth in the presence of a wild-typeS. douglasii mitochondrial genome, but not in the presence of an intronlessS. cerevisiae mitochondrial genome. Also theS. douglasii andS. cerevisiae CBP2 genes are completely interchangeable, even though the intron bI5 is absent from theS. douglasii mitochondrial genome. |
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| Keywords: | Saccharomyces douglasii Saccharomyces cerevisiae CBP2 Mitochondria Pre-mRNA processing |
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