The lipopolysaccharide of the green sulfur bacterium Chlorobium vibrioforme f. thiosulfatophilum |
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Authors: | J Meißner U Fischer J Weckesser |
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Institution: | (1) Institut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität, Schänzlestrasse 1, D-7800 Freiburg i. Br., Germany;(2) Fachbereich Biologie, AG Geomikrobiologie, der Universität Oldenburg, Postfach 2503, D-2900 Oldenburg, Germany |
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Abstract: | The cell wall lipopolysaccharide of the green sulfur bacterium Chlorobium vibrioforme f. thiosulfatophilum was obtained by the phenol-chloroform-petroleum ether and the hot phenol-water methods, respectively. It contained mannose, glucose, galacturonic acid, glucosamine, glycine, and small amounts of rhamnose, galactose and glucuronic acid. In addition to d-glycero-d-mannoheptose, the corespecific constituents 2-keto-3-deoxyoctonate and l-glycero-d-mannoheptose were found. Polyacrylamide gel-electrophoresis in the presence of sodium deoxycholate gave no indication for the presence of O-specific repeating units. Degradation of the lipopolysaccharide required 10% acetic acid (100° C, 2 h). The lipid A moiety contained the total of glucosamine of the lipopolysaccharide as well as small amounts of 2,3-diamino-2,3-dideoxy-glucose. It was phosphate-free. The fatty acid spectrum comprised 3-OH-14:0, 3-OH-16:0, and iso-3-OH-18:0 besides little 12:0, 14:0 and 16:0. Hydroxylaminolysis and sodium methylate treatment revealed all of the three hydroxy fatty acids to be amidebound.Abbreviations DOC
sodium deoxycholate
- PAGE
polyacrylamide gel-electrophoresis |
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Keywords: | Chlorobiaceae Chlorobium vibrioforme Degraded polysaccharide DOC-PAGE Lipid A Lipopolysaccharide |
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