Fermentation of xylose and rice straw hydrolysate to ethanol byCandida shehatae NCL-3501 |
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Authors: | M Abbi R C Kuhad Dr A Singh |
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Institution: | (1) Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, 110021 New Delhi, India;(2) Microbial Biotechnology Laboratory, Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada;(3) Present address: Department of Biochemistry and Molecular Biology, University of Georgia, 30602-7229 Athens, Georgia, USA |
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Abstract: | Candida shehatae NCL-3501 utilized glucose and xylose efficiently in batch cultures. The specific rate of ethanol production was higher with mixtures of glucose and xylose (0.64–0.83 g g–1 cells d–1) compared to that with individual sugars (0.38–0.58 g g–1 cells d–1). Although the optimum temperature for growth was 30°C, this strain grew and produced appreciable levels of ethanol at 45°C. A stable ethanol yield (0.40–0.43 g g–1 substrate utilized) was obtained between 10 g L–1 and 80 g L–1 of initial xylose concentration. Conversion efficiency was further improved by immobilization of the cells in calcium alginate beads. Free or immobilized cells ofC. shehatae NCL-3501 efficiently utilized sugars present in rice straw hemicellulose hydrolysate, prepared by two different methods, within 48 h. Ethanol yields of 0.45 g g–1 and 0.5 g g–1 from autohydrolysate, and 0.37 g g–1 from acid hydrolysate were produced by free and immobilized cells, respectively. |
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Keywords: | Candida shehatae xylose rice straw hydrolysate ethanol immobilized cells |
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