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A new buffer system for acid PAGE typing of equine protease inhibitor
Authors:P. J. Henney  E. L. Johnson  E. G. Cothran
Affiliation:Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA;Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA;Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA
Abstract:A new buffer system for acid PAGE typing of protease inhibitor (Pi) is described. This buffer system replaces pyridine and cacodylic acid with L-histidine and MES, making the buffer less toxic and less expensive than the acid PAGE system commonly used but with no loss of resolution.
Keywords:protease inhibitor    electrophoresis    PAGE
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