欧李DAM5基因的克隆及表达分析

休眠相关MADS-box(DAM)基因在植物芽休眠诱导过程中发挥重要作用。该研究以欧李‘农大4号’不同阶段的芽为材料,利用PCR技术,克隆获得ChDAM基因。ChDAM基因全长705 bp,编码234个氨基酸。多序列比对与结构域分析发现,ChDAM基因编码的蛋白具有典型的MADS-box结构域和K-box结构域,其氨基酸序列与桃的DAM5蛋白相似性较高。启动子分析发现,ChDAM5基因的表达可能会受到低温和脱落酸的调控。实时荧光定量PCR分析发现,ChDAM5基因的表达随着休眠诱导进程逐渐升高。研究推测,ChDAM基因在诱导欧李芽休眠中发挥关键作用,为后续深入的代谢途径研究奠定了基础。

Cloning and Expression Analysis of a DAM5 Gene in Cerasus humilis

The dormancy associated MADS-box(DAM)gene plays an important role in the induction of plant bud dormancy.In this study,we used the different stages of Cerasus humilis‘Nongda 4’as materials to obtain the ChDAM gene using PCR technology.The gene has a total length of 705 bp and encodes 234 amino acids.Multi-sequence comparison and domain analysis found that the gene encoded protein has a typical MADS-box domain and K-box domain,and its amino acid sequence is more homologous to peach DAM5 protein.The promoter analysis found that the expression of the ChDAM5 gene may be regulated by low temperature and abscisic acid.Real-time quantitative PCR results found that the expression of the ChDAM5 gene gradually increased with the process of dormancy induction.So it was speculated that the gene played a key role in inducing C.humilis dormancy,laying the foundation for further in-depth metabolic pathway research.