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Pulicaria crispa mitigates gastric ulcer induced by ethanol in rats: role of treatment and auto healing |
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| Authors: | Abdelgawad A. Fahmi Mariam Abdur-Rahman Asmaa F. Aboul Naser Howaida I. Abd-Alla Mohamed I. Nasr |
| Affiliation: | 1. Faculty of Science, Chemistry Department, Cairo University, Cairo, Egypt;2. Department of Therapeutic Chemistry, National Research Centre, Giza, Egypt;3. Chemistry of Natural Compounds Department, National Research Centre, Giza, Egypt;4. Clinical Pathology Laboratory, Cairo Specialized Hospital, Cairo, Egypt |
| Abstract: | Context: Stomach ulcers are the common gastrointestinal disorders worldwide. Objective: This study aimed to investigate the therapeutic impact of Pulicaria crispa aerial parts ethanol extract against gastric ulcer in rats. Materials and methods: Ulcer was induced by one oral dose of ethanol (0.5?ml/100g body weight) on 24?hours empty stomach, then the plant extract (500?mg/kg b.wt.) was orally administered daily for one week. Ranitidine (100?mg/kg b.wt.); as a reference drug was evaluated. Stomach acidity and volume, as well as lesion counts were measured. Levels of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were estimated. Assay of different marker enzymes; succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glucose-6-phosphatase (G-6-Pase), acid phosphatase (AP) and 5′-nucleotidase (5′NT) were determined. Interlukin-10 (IL-10), intracellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor alpha (TNF-α) were also determined. Stomach histopathological assessment was detected. Results: Gastric ulcer showed drastic changes in oxidative stress, cell organelles and inflammatory markers. These biomarkers served as good tools to identify the presence of gastric ulcer. Treatment with P. crispa recorded amelioration in most parameters exceeding the auto healing effect. Conclusion: Healing potency of P. crispa is possibly related to its content of glycosides, coumarins, flavonoids, tannins, sterols and triterpenes. |
| Keywords: | Gastric ulcer ethanol Pulicaria crispa enzymes antioxidant inflammatory mediators histopathology |