Uncinaria stenocephala: assessment of antigens for the immunodiagnosis of canine uncinariosis |
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Authors: | Postigo I Martinez J Guisantes J A |
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Institution: | Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain. |
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Abstract: | Although Uncinaria stenocephala is the most frequent hookworm in the intestine of dogs from Northern, Central and Southern Europe, little is known about its host-parasite relationship. Three groups of sera from dogs (Group 1: dogs naturally infected only by U. stenocephala; Group 2: helminth-free dogs at necropsy, and Group 3: dogs parasitized by other helminths) were analyzed by ELISA using U. stenocephala antigens from adult worms (somatic and excretory-secretory antigens) and from L3 larvae (somatic antigens). All three sources of antigens were found to be suitable for immunodiagnosis of canine uncinariosis with up to 90% efficacy. However, an analysis to assess the diagnostic value of the different antigens demonstrated that the adult excretory-secretory antigens had a higher diagnostic efficacy (96.7%), indicating that this is the best antigen source for the diagnosis of Uncinaria infection. |
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Keywords: | Uncinaria stenocephala Nematode Hookworm Dog uncinariosis Parasite antigen Larval antigen L3 infective larva PBS phosphate-buffered saline AE-S adult excretory-secretory antigens AS adult somatic antigens L3S larval somatic antigens BSA bovine seroalbumin ROC receiver operating characteristic ELISA enzyme-linked immunosorbent assay |
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