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A cytokinin glucoside from the leaves of Phaseolus vulgaris L.
Authors:T. L. Wang  A. G. Thompson  R. Horgan
Affiliation:(1) Department of Botany and Microbiology, The University College of Wales, Penglais, SY23 3DA Aberystwyth, UK;(2) Present address: Botany Department, University of Leicester, LE1 7RH Leicester, UK;(3) Present address: The Polytechnic, Holly Bank Road, HD3 3BP Huddersfield, UK
Abstract:
Phaseolus vulgaris plants decapitated above the primary leaves accumulate high cytokinin activity. The major cytokinin in these leaves was identified by Sephadex LH 20 chromatography, sensitivity to beta-glucosidase and permanganate oxidation, and by combined gas chromatography-mass spectrometry as 6-(4-O-beta-D-glucosyl-3-methylbutylamino) purine, (dihydrozeatin-O-beta-D-glucoside). A possible reason for the persistance of this compound in the primary leaves is discussed.Abbreviations BSA Bis-(trimethylsilyl)-acetamide - DHZ dihydrozeatin - DHZOG dihydrozeatin-O-beta-D-glucoside - TMS trimethylsilyl - Z zeatin
Keywords:Cytokinin  Leaves  Phaseolus
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