Calcium transport sensitive to ruthenium red in cytochrome oxidase vesicles reconstituted with mitochondrial proteins |
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| Authors: | Cecilia Zazueta José A Holguín Jorge Ramírez |
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| Institution: | (1) Departamento de-Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, 041080 México, D.F., México;(2) Instituto de Fisiología Celular, Universidad Nacional Autonoma de México, Departamento de Microbiología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado 70-600, 04510, D.F. México, México |
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| Abstract: | We describe a calcium transport that is sensitive to ruthenium red in liposomes reconstituted with mitochondrial extracts. This system is able to build an internally negative membrane potential, which allows the electrogenic influx of Ca2+ and Sr2+. Proteins with molecular weights higher than 35 kDa were incorporated to the vesicles, and enhanced the accumulation of the cation in an energy-dependent fashion. |
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| Keywords: | Ca2+ transport liposomes ruthenium red mitochondria |
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