| DIG标记DNA探针检测noggin在爪蟾胚胎中的表达 |
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| 引用本文： | 周文,冯湘玲,杨旭宇,王磊,姚开泰.DIG标记DNA探针检测noggin在爪蟾胚胎中的表达[J].生命科学研究,2002,6(3):239-242. |
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| 作者姓名： | 周文 冯湘玲 杨旭宇 王磊 姚开泰 |
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| 作者单位： | 中南大学,湘雅医学院,肿瘤研究所,中国湖南,长沙,410078 |
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| 基金项目： | 国家自然科学基金资助项目 ( 39910 16 1994) |
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| 摘 要： | 整体原位杂交（Whole-mount in situ hybridization)用于基因表达定位和表达分布模式的研究，已经成为一种非常重要的手段。PCR方法进行探针标记，可以获得特异性高，片断大小可变的探针。采用DIG标记，检测已知基因noggin在爪蟾胚胎时空分布，所得结果与文献报道一致，表明PCR方法获得的DNA探针在一定的条件下可以用于爪蟾胚胎整体原位杂交。
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| 关 键 词： | DIG标记 DNA探针 检测 noggin 爪蟾胚胎 表达 整体原位杂交 |
| 文章编号： | 1007-7847(2002)03-0239-04 |
| 修稿时间： | 2002年4月16日 |
Detection of Expression Distribution of Noggin in Xenopus Embryos with DIG-labeled DNA Probe |
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| ZHOU Wen,FENG Xiang-ling,YANG Xu-yu,WANG Lei,YAO Kai-tai.Detection of Expression Distribution of Noggin in Xenopus Embryos with DIG-labeled DNA Probe[J].Life Science Research,2002,6(3):239-242. |
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| Authors: | ZHOU Wen FENG Xiang-ling YANG Xu-yu WANG Lei YAO Kai-tai |
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| Abstract: | Whole-mount in situ hybridization has become a very important tool for studying localization and distribution pattern of gene expression.Highly specific probes were obtained,the length of which are variable.With DIG labeling,the temporal and spatial expression of noggin,one of known genes,was detected.The results obtained were consistent with those in paper previously reported,suggesting that the DNA probe produced by PCR is suitable for whole-mount in situ hybridization on Xenopus embryos under specific conditions. |
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| Keywords: | whole-mount in situ hybridization DNA probe Xenopus noggin |
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