滴鼻途径建立鼠结核病模型的探讨

目的 探讨滴鼻途径建立BALB/C小鼠结核分枝杆菌感染的模型的可行性.方法 人型Mtb H_(37)Rv标准株经腹腔接种小鼠,取小鼠腹腔冲洗液100 μl接种改良罗-琴氏培养基.刮取上述培养基上生长4周已恢复毒力的结核分枝杆菌H_(37)Rv标准株,加0.05%Tween80生理盐水磨菌制成悬液,菌落计数,计数后稀释悬液为5×10~3 CFU/50 μl、5×10~4 CFU/50 μl、5×10~5 CFU/50 μl及50 μl生理盐水分别感染4组Balb/c小鼠,制作结核分枝杆菌感染模型.结果 滴鼻感染小鼠4周后,所有小鼠肺、脾组织中均可见抗酸阳性菌,在感染小鼠肺、脾组织匀浆均培养出Mtb.肺组织病理改变明显,正常肺泡结构消失,以充血实变、淋巴细胞、巨噬细胞浸润为主,增生性改变不明显,未见明显的组织坏死.脾组织病理改变主要是巨噬细胞和淋巴细胞增生.结论 滴鼻感染途径建立小鼠结核病模型简便、可行,为进一步研究开发重组BCG疫苗对鼠结核病的防治打下良好的基础. 0~4 CFU/50 μl、5×10~5 CFU/50 μl及50 μl生理盐水分别感染4组Balb/c小鼠,制作结核分枝杆菌感染模型.结果 滴鼻感染小鼠 周后,所有小鼠肺、脾组织中均可见抗酸阳性菌,在感染小鼠肺、脾组织匀浆均培养出Mtb.肺组织病理改变明显,正常肺泡结构消失,以充血实变、淋巴细胞、巨噬细胞浸润为主,增生性改变不明显,未见明显的组织坏死.脾组织病理改变主要是巨噬细胞和淋巴细胞增生.结论 滴鼻感染途径建立小鼠结核病模型简便、可行,为进一步研究开发重组BCG疫苗对鼠结核病的防治打下良好的基础. 0~4 CFU/50 μl、5×10~5 CFU/50 μl及50 μl生理盐水分别感染4组Balb/c

Establishment of A Mouse Tuberculosis Model for Mycobacterium tuberculosis Intranasal Infection

Objective To establish a mouse tuberculosis model for Mycobacterium tuberculosis (Mtb) infection in intranasal. Methods The virulent strain Mtb H37Rv was passed through mice and cultured on Lowenstein-Jensen media for 4 weeks, then harvested and diluted to 5×10~3 CFU/50 μl, 5×10~4 CFU/50 μl and 5×10~5CFU/50 μl in saline containing 0.05% Tween80. Balb/c mice were infected with 50 μl normal saline, 5×10~3CFU/50 μl Mtb, 5×10~4CFU/50 μl Mtb, or 5×10~5 CFU/50 μl Mtb. Result Mycobacteria were found in the lungs and spleens by acid-fast staining in all mice infected with Mtb H37Rv. The bacteria were cultured on Lowenstein-Jensen media from lung and spleen tissue. The lesions on the lungs were excessive and extensive, alveoli and interalveolar septae were effaced by infiltrates of macrophages and lymphocytes, but necrosis was not found. The proliferation of macrophages and lymphocytes were founded in spleens tissue. Conclusion The tuberculosis model for Mycobacterium tuberculosis infection in intranasal was established successfully. The model provides an experimental basis for further study of the immunoprotection of recombinant BCG after Mycobacterium tuberculosis infected.