Chemical composition and antimicrobial activity of Citrus essences on honeybee bacterial pathogen <Emphasis Type="Italic">Paenibacillus larvae</Emphasis>, the causal agent of American foulbrood |
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Authors: | Sandra R Fuselli Susana B García de la Rosa Martín J Eguaras Rosalía Fritz |
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Institution: | (1) Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350 (7600), Mar del Plata, Argentina;(2) Comisión de Investigaciones Científicas (CIC), La Plata, Argentina;(3) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina |
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Abstract: | Antimicrobial properties and chemical composition of four citrus fruit essential oils to control Paenibacillus larvae, the causal agent of American foulbrood disease (AFB) were determined. This honeybee larvae disease occurs throughout the
world and is found in many beekeeping areas of Argentina. Citrus fruit essential oils tested were those from grapefruit (Citrus paradisi), sweet orange (Citrus sinensis), mandarin (Citrus nobilis) and lemon (Citrus limon). The components of the essential oils were identified by SPME-GC/MS analysis. The antimicrobial activity of the oils against
P. larvae were determined by the broth microdilution method. Two way ANOVA tests for minimum inhibitory concentrations (MICs) data
and minimal bactericide concentrations (MBCs) data, indicated significant differences between the strains and the oils tested.
The antimicrobial assays showed that the oil of C. paradisi inhibited the bacterial strains at the lowest concentrations tested, MICs and MBCs averages of 385.0 mg/l and 770.0 mg/l,
respectively. This property could be attributed to the kind and percentage of the volatile components of the oil, like limonene
(69.9%) and myrcene (9.6%). The use of essential oils or their specific volatile components individually against pests related
to food provision may represent an alternative scope for the control of this serious disease because it does not leave toxic
chemical residues in honey nor in its by products. |
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Keywords: | Citrus fruit essential oils Chemical composition GC-MS Antimicrobial activity Paenibacillus larvae American foulbrood Honeybees |
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