Periplasmic superoxide dismutases in Aquaspirillum magnetotacticum |
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| Authors: | K A Short R P Blakemore |
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| Institution: | (1) Department of Immunology and Microbiology, University of Michigan Medical School, 6643 Medical Science Building II, 48109-0620 Ann Arbor, MI, USA;(2) Department of Microbiology, University of New Hampshire, Spaulding Life Sciences Building, 03824 Durham, NH, USA |
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| Abstract: | Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytoplasm. Irontype SOD (FeSOD) contributed 88% of the total activity activity detected, although a manganese-type SOD (MnSOD) was present in the periplasm as well. Cells cultured at a higher dissolved O2 tension (10% of saturation) expressed increased activity of the MnSOD relative to that of the FeSOD. |
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| Keywords: | Magnetic bacteria Periplasm Superoxide dismutase Peroxidase Microaerophiles O2 toxicity Aquaspirillum magnetotacticum |
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