A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae. |
| |
| Authors: | R W Heiderpriem P D Livant E J Parish R J Barbuch M G Broaddus M Bard |
| |
| Institution: | Department of Chemistry, Auburn University, AL 36849. |
| |
| Abstract: | A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component. |
| |
| Keywords: | |
|
|
