细叶黄芪叶肉原生质体发育早期几种细胞器的变化

在细叶黄芪叶肉原生质体发育早期,细胞器的变化较大。离体培养4h后,线粒体的嵴和基质物质开始增加。培养3—5天后,线粒体的数量增加5倍以上,此时可见大部分线粒体围绕细胞核分布。在培养24h后,高尔基体开始发育,它们主要分布在细胞质周边区域。多糖细胞化学染色表明,高尔基体内沉积着大量嗜银物质。培养1天后,粗面内质网开始发育。培养3天时,部分叶绿体边缘出现一些空隙结构。随着叶绿体内膜结构的消失,淀粉粒增大,叶绿体逐渐转变为造粉质体。

THE CHANGES OF SOME ORGANELLES OCCURRED IN THE EARLY DEVELOPMENTAL STAGES OF THE MESOPHYLL PROTOPLASTS OF ASTRAGALUS MELILOTOIDES VAR. TENUIS

Dramatic changes of some organelles were observed during the early developmental stages of mesophyll protoplasts from A. melilotoides var. tennis. After 4 h in culture, the number of cristae and the quantity of matrix in mitochondria began to increase. A more than 5-fold increase in the number of mitochondria was reached after 3-5 days in culture, and the mitochondria-were mainly distributed around the cell nucleus. In 24 h protoplasts, the Golgi bodies, which were located at the periphery of cytoplasm, began to develop. The cytochemical staining specific to poly-saccharide showed that massive silverophi-lic material was accumulated within Golgi bodies. After l day in culture, the rough endoplasmic reticulum (RER) has remarkably increased in quantity. In addition, proplastids were seen "in a number of protoplasts at day 3. In chloroplasts of 3 day protoplasts, many cavities occurred at their peripheries. The starch grains became conspicuous in chloroplasts when the inner membrane structures were disorganized, and the chloroplasts began to turn into amyloplasts. Problems of the functions of structural changes of mitochondria, endoplasmic reticulum, Golgi bodies and chloroplasts in protoplast development were discussed in this paper.