Subcellular localization of enzymes of anthocyanin biosynthesis in protoplasts |
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| Authors: | Geza Hrazdina George J. Wagner Harold W. Siegelman |
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| Affiliation: | 1. Cornell University, Department of Food Science and Technology, New York State Agricultural Experiment Station, Geneva, NY 14456 U.S.A.;2. Biology Department, Brookhaven National Laboratory, Upton, NY 11973, U.S.A. |
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| Abstract: | ![]()
Flavanone synthase, chalcone-flavanone isomerase and UDP-glucose; anthocyanidin-3-O-glucosyltransferase activities of protoplasts and subcellular fractions of protoplasts of Hippeastrum and Tulipa were investigated. Subcellular fractions studied were intact vacuoles, cytosol and particulate components of protoplasts less the vacuole. The cytosol fraction had the highest activity of the three enzymes studied. Results similar to those found for Hippeastrum were obtained with fractions from leaves and petals of Tulipa. The increase in flavanone synthase activity in the cytosol fraction from petals of Hippeastrum during development paralleled the increase in anthocyanin content of the petals. |
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| Keywords: | Liliaceae enzymes of anthocyanin biosynthesis subcellular localization protoplasts. |
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