Differential Giemsa staining in plants

Interference contrast and scanning electron microscopy studies of BSG treated Tulipa somatic chromosomes revealed transverse ridges coincident with the Giemsa positive areas. No cytological differentiation of the Giemsa banded regions was observed either prior to staining, or following destaining in methanol. Thus, the ridges observed must be interpreted as a result of an accumulation of dye molecules rather than a differential distribution of chromosomal material either before or after staining.