Protein adsorption and displacement at lipid layers determined by total internal reflection fluorescence (TIRF) |
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| Authors: | Lene Jorgensen Grith Krøyer Wood Ida Rosenkrands Charlotte Petersen Dennis Christensen |
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| Institution: | 1. Department of Pharmaceutics and Analytical Chemistry, The Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmarklej@farma.ku.dk;3. Vaccine development, Statens Serum Institut, Copenhagen, Denmark;4. Vaccine delivery &5. formulation, Dept. of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark;6. Department of Pharmaceutics and Analytical Chemistry, The Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark |
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| Abstract: | In many drug delivery systems such as liposomes, the adsorption of interstitial proteins upon administration can have a huge effect on the elimination, release, and stability of the delivery system. For example, it is assumed that PEGylated liposomes prevent the adsorption of opsonins and thereby prolong the circulation time in vivo, and EMEA guidelines recommend that more than 80% of the protein antigen is adsorbed in the formulation of adjuvant systems. However, few methods exist to elucidate this protein adsorption. The present study indicates that total internal reflection fluorescence (TIRF) is a possible method to examine the adsorption and exchange of proteins at lipid surfaces. In the TIRF set-up, a lipid layer can be formed exemplified with dimethyldioctadecylammonium bromide (DDA) and D-(+)-trehalose 6,6’-dibehenate (TDB)] whereafter protein (i.e., ovalbumin or an antigen, Ag85B-ESAT-6) is adsorbed, and these proteins can subsequently be displaced by the abundant interstitial protein (i.e., serum albumin). |
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| Keywords: | Protein adsorption total internal reflection fluorescence TIRF lipid layer liposomes vaccine |
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