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In vitro fertilization of pig oocytes matured in vitro
Authors:M. Betancourt   R. Fierro  D. Ambriz
Affiliation:

Departamento de Ciencias de la Salud Universidad Autónoma Metropolitana-Iztapalapa C.P. 09340, México D.F., México

Abstract:The development of in vitro fertilization (IVF) techniques in pigs as well as in other species is of great importance because of the possible applications of this technology in different research fields. Methods of IVF vary in different incubation periods and temperatures, in the hormone concentrations used, and in the treatment of the sperm samples. It has been particularly difficult to succeed in the achievement of fertilization in the pig. In the present study we used FSH and LH concentrations of 2 IU/ml for oocyte maturation, an incubation temperature of 37°C, and dilution of spermatozoa for capacitation, and we achieved a high fertilization rate (50 to 75%) with no cases of polyspermy.
Keywords:porcine ova   in vitro maturation   fertilization   capacitation   spermatozoa
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