Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph |
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Authors: | Ji Eun Kim Eun Jeong Kim Won Jong Rhee Tai Hyun Park |
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Institution: | (1) School of Chemical and Biological Engineering, Seoul National University, 151-744 Seoul, Korea |
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Abstract: | The effect of silkworm hemolymph on the expression of recombinant protein inEscherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase
inE. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in
media supplemented with 1,3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph
was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified
as the effective component. |
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Keywords: | E coli recombinant protein silkworm hemolymph |
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