| 高速逆流色谱分离制备川西獐牙菜中两种苷类化合物 |
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| 引用本文： | 陈涛,贾静,王萍,尤进茂,刘永军,李玉林.高速逆流色谱分离制备川西獐牙菜中两种苷类化合物[J].天然产物研究与开发,2012,24(6):800-803,859. |
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| 作者姓名： | 陈涛 贾静 王萍 尤进茂 刘永军 李玉林 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,西宁810001;中国科学院研究生院,北京100049
2. 中国科学院西北高原生物研究所,西宁,810001 |
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| 基金项目： | 国家自然科学基金项目，中国科学院知识创新工程重要方向项目 |
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| 摘 要： | 采用高速逆流色谱从川西獐牙菜中分离制备了两种高纯度苷类化合物.以正丁醇-氯仿-甲醇-水(3.4∶8∶5∶6,v/v)为溶剂系统,主机转速为800 rpm,流速:O～210 min,1.5mL/min;210 ～360m in,2.5 mL/min,检测波长254 nm的条件下进行分离制备,在360 min内从100 mg样品中一步分离制备得到1-O-樱草糖-3,7,8-三甲氧基(口山)酮(Ⅰ,11 mg)和异荭草苷(Ⅱ,24 mg).经HPLC检测,两个化合物的纯度均在99％以上,结构由UV、1H和13C NMR鉴定.
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| 关 键 词： | 川西獐牙菜 高速逆流色谱 1-O-樱草糖-3 7 8-三甲氧基(口山)酮 异荭草苷 |
Preparative Separation of Two glycosides from Swertia Mussotti by High-Speed Counter-Current Chromatography |
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| CHEN Tao
,
JIA Jing
,
WANG Ping
,
YOU Jin-mao
,
LIU Yong-jun
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LI Yu-lin.Preparative Separation of Two glycosides from Swertia Mussotti by High-Speed Counter-Current Chromatography[J].Natural Product Research and Development,2012,24(6):800-803,859. |
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| Authors: | CHEN Tao JIA Jing WANG Ping YOU Jin-mao LIU Yong-jun LI Yu-lin |
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| Institution: | 1* 1Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China; 2Graduate School of the Chinese Academy of Sciences,Beijing 100049,China |
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| Abstract: | A high performance method for isolation and purification of two glycosides from Swertia mussotti was successfully established by utilizing high-speed counter-current chromatography(HSCCC).Separation was performed with a two-phase solvent system composed of n-butanol-chloroform-methanol-water(3.4 ∶8 ∶5 ∶6,v/v).1-O-primeverosyl-3,7,8-trimethoxyxanthone(11 mg) and isoorientin(24 mg) were isolated and purified in one-step elution from 100 mg crude sample under the conditions of a flow rate 1.5 mL/min in the initial 210 min followed by 2.5 mL/min,800 r/min and the detection wavelength of 254 nm.HPLC analysis showed that the purity of two compounds was over 99%.Their chemical structures were confirmed by UV,1H-and 13C NMR. |
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| Keywords: | Swertia mussotti HSCCC 1-O-primever-osyl-3 7 8-trimethoxyxanthone isoorientin |
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