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白背三七大孔树脂富集物化学成分及抗氧化活性研究
引用本文:接敏,万春鹏,余燕影,曹树稳. 白背三七大孔树脂富集物化学成分及抗氧化活性研究[J]. 天然产物研究与开发, 2012, 24(2): 155-159,163
作者姓名:接敏  万春鹏  余燕影  曹树稳
作者单位:1. 南昌大学食品科学与技术国家重点实验室,南昌,330047
2. 南昌大学化学系,南昌,330031
3. 南昌大学食品科学与技术国家重点实验室,南昌330047;南昌大学化学系,南昌330031
摘    要:
本文采用HPLC-ESI-MS分析白背三七醇提液经HPD-100树脂富集分离后的黄酮类化合物,并采用化学和生物模型对富集物的抗氧化活性进行研究.实验结果表明该富集物主要黄酮成分为槲皮素和山奈酚的糖苷类化合物,且以山奈酚-3 -O-β-D-葡萄糖苷的含量最高;白背三七醇提液经富集后总黄酮含量提高了6倍.在试验模型中,除螯合能力外,富集后产物抗氧化能力较富集前均有显著提高,说明黄酮类化合物是白背三七抗氧化活性的主要物质基础.

关 键 词:白背三七  黄酮类化合物  化学成分  抗氧化活性

Chemical Constituents and Antioxidant Activity of Gynura divaricata Extract Enriched by Macroporous Resin
JIE Min , WAN Chun-peng , YU Yan-ying , CAO Shu-wen. Chemical Constituents and Antioxidant Activity of Gynura divaricata Extract Enriched by Macroporous Resin[J]. Natural Product Research and Development, 2012, 24(2): 155-159,163
Authors:JIE Min    WAN Chun-peng    YU Yan-ying    CAO Shu-wen
Affiliation:1,2 1State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2Department of Chemistry,Nanchang University,Nanchang 330031,China
Abstract:
HPLC-ESI-MS method was applied to analyze and identify the flavonoids from Gynura divaricata extract,which was enriched by HPD-100 macroporous resin,and the antioxidant activity of extract was investigated by biological and chemical models.The results showed that quercetin and kaempferol glycosides were the main constituents in G.divaricata extract and the content of kaempferol-3-O-β-D-glucoside was the highest.After treating with HPD-100 resin,the content of flavonoids increased more than 6 times,and the antioxidant activity improved greatly except the chelating ability,which indicated that the flavonoids compounds in G.divaricata extract were the main substance of antioxidant activity.
Keywords:Gynura divaricate  flavonoids  chemical constituents  antioxidant activity
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