虎眼万年青属植物Ornithogalum thyrsoide 的甾体皂苷及黄酮类化学成分

运用硅胶、Sephadex LH-20、ODS柱层析及半制备高效液相色谱技术对一引种虎眼万年青属植物Ornithogalum thyrsoides的化学成分进行研究,从其乙醇提取物的乙酸乙酯部分分离得到7个化合物.通过MS、NMR等波谱技术确定其结构分别为(23S,24S,25S)-23,24-dihydroxyruscogenin- 1-O-α-L-rhamnopyranosyl(1 →2)-α-L-arabinopyranoside(1),(24S,25S)-3β,24-dihydroxyspirost-5 -en- 1β-yl O-α -L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside(2),β-胡萝卜苷(3)β-谷甾醇(4),山奈酚-4′-O-β-D-吡喃葡萄糖苷(5),千层纸素A(6)和汉黄芩素(7).其中,化合物1、5～7为首次从该属植物中分离得到.体外药理活性结果显示这7个化合物对HCT-8、Bel-7402、BGC-823、A549及A2780实体瘤细胞株无明显细胞毒活性.

Steroidal Saponins and Flavonoids from Ornithogalum thyrsoides

Seven compounds were isolated and purified by silica gel,Sephadex LH-20,ODS and semi-preparative HPLC from ethyl acetate extraction of Ornithogalum thyrsoides.By means of extensive spectroscopic data,their structures were identified as(23S,24S,25S)-23,24-dihydroxyruscogenin 1-O-α-L-rhamnopyranosyl-(12)-α-L-arabinopyranoside(1),(24S,25S)-3β,24-dihydroxyspirost-5-en-1β-yl O-α-L-rhamnopyranosyl-(12)-α-L-arabinopyranoside(2),β-daucosterol(3),β-sitosterol(4),kaempferol-4′-O-β-D-glycopyranoside(5),oroxylin A(6),and wogonin(7).Compounds 1,5-7 were isolated from this genus for the first time.All compounds were evaluated in vitro for their cytotoxicities,but none of them showed significant cytotoxic activity against five tumor cell lines HCT-8,Bel-7402,BGC-823,A549 and A2780.