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Proteolytic activity in the digestive fluid of larvae of Trichoplusia ni
Affiliation:1. School of Chemical and Process Engineering (SCAPE), University of Leeds, Leeds LS2 9JT, UK;2. Centre for Doctoral Training in Bioenergy, University of Leeds, Leeds LS2 9JT, UK;3. Centre for Integrated Energy Research, University of Leeds, Leeds LS2 9JT, UK;4. EDF Energy, West Burton Power Station, Retford, Nottinghamshire DN22 9BL, UK
Abstract:
The digestive fluid of Trichoplusia ni larvae has caseinolytic activity over a wide alkaline range, with greatest activity at pH 9.8. Activity was linear with respect to time and concentration. The temperature optimum is 45°C. Tryptic and chymotryptic activities were demonstrated using the substrates TAME and BTEE and the inhibitors TLCK, TPCK, and PMSF.
Keywords:
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