Distribution of high mobility group and other acid-soluble proteins in fractionated chromatin |
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Affiliation: | 1. Department of Energy and Environment Convergence, Catholic Kwandong University, Beomil-ro 579, Gangneung 210-701, Korea;2. Department of Environmental Engineering, Catholic Kwandong University, Beomil-ro 579, Gangneung 210-701, Korea;3. Department of Civil Engineering, Catholic Kwandong University, Beomil-ro 579, Gangneung 210-701, Korea;1. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore;2. IBM Almaden Research Center, 650 Harry Road, San Jose, CA 95120, USA |
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Abstract: | Chromatin was fractionated by digestion with deoxyribonuclease II and precipitation with MgCl2. The Mg2+-soluble fraction, known to be enriched in transcribed DNA sequences, was enriched also in high mobility group proteins 1 and 2 and contained almost all other acid-soluble nonhistone proteins. |
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