Rapid vegetative multiplication in Colocasia esculenta (L) Schott (taro) |
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Authors: | H. Chand M. N. Pearson |
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Affiliation: | (1) Plant Science Research Group, School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand |
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Abstract: | ![]() Initial multiplication of taro was achieved by dividing corms into pieces, each with several buds, and planting them in sterile potting mix. Surface planting and decapitation of the new primary meristem on each sucker forced additional shoot meristems to grow. Meristems of six cultivars were cultured on a modified Murashige and Skoog medium plus TDZ. In experiments with the cultivar Niue, explants cultured on modified MS medium plus 2.6 μM (0.6 mg L-1 TDZ grew more vigorously than on media including BA. Transfer to 4.3 μM (1.0 mg L-1 TDZ subsequently gave a 15–25 fold increase in production of plantlets per four week culture period compared to four-fold with BAP. The two stage propagation technique described in this paper is an efficient way to produce taro plants for commercial planting or the production of pathogen-tested material. This revised version was published online in June 2006 with corrections to the Cover Date. |
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