Preparation and characterization of monoclonal antibodies which recognise different gibberellin epitopes |
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| Authors: | J. P. Knox M. H. Beale G. W. Butcher J. MacMillan |
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| Affiliation: | (1) Monoclonal Antibody Centre, Institute of Animal Physiology, Babraham, CB2 4AT Cambridge, UK;(2) School of Chemistry, University of Bristol, BS8 1TS Bristol, UK |
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| Abstract: | The production and characterization of high-affinity monoclonal antibodies (McAb) to gibberellins (GAs) is reported. Hybrid myelomas were derived from immunisations with conjugates in which immunogenic proteins were linked to GA1 at carbon-3 and to GA4 and GA9 at carbon-17. A series of McAb which display specificities allowing recognition of, and the discrimination between GA1, GA20, GA4 and GA9 is described. These McAb can be used in quantitative immunoassays for underivatised GAs.Abbreviations BSA bovine serum albumin - FCS foetal calf serum - GAn gibberellin An - IFA incomplete Freund's adjuvant - KLH keyhole-limpet haemocyanin - McAb monoclonal antibody (bodies) - PBS phosphate-buffered saline |
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| Keywords: | Gibberellin (monoclonal antibodies, structure specificity) Immunoassay Monoclonal antibody |
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