| 我国真菌传线状小麦花叶病毒病病原初步鉴定为小麦黄花叶病毒(WYMV) |
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| 引用本文： | 雷娟利,陈炯,陈剑平,郑滔,程晔. 我国真菌传线状小麦花叶病毒病病原初步鉴定为小麦黄花叶病毒(WYMV)[J]. Virologica Sinica, 1998, 0(1) |
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| 作者姓名： | 雷娟利 陈炯 陈剑平 郑滔 程晔 |
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| 作者单位： | 浙江省农业科学院 |
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| 基金项目： | 欧共体INCO项目,863项目,浙江省青年科技人才资金 |
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| 摘 要： | 由禾谷多粘菌（Ｐｏｌｙｍｙｘａｇｒａｍｉｎｉｓ）传播的线状小麦花叶病毒有两种，一种是加拿大首先报道的小麦梭条斑花叶病毒（ＷＳＳＭＶ），另一种是日本报道的小麦黄花叶病毒（ＷＹＭＶ）。这两种病毒粒子形态以及血清学性质非常相似，但核酸序列存在一定差异。经反转录聚合酶链反应（ＲＴＰＣＲ）和单链构象多态性分析（ＳＳＣＰ），明确我国广泛发生的禾谷多粘菌传线状小麦花叶病毒都是小麦黄花叶病毒，但供试的８个分离物ＲＮＡ１和ＲＮＡ２序列存在差异，无一彼此完全相同
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| 关 键 词： | 真菌传线状小麦花叶病毒，反转录-聚合酶链反应，单链构象多态性，小麦黄花叶病毒 |
Identification of Chinese Fungal transmitted Filamentous Wheat Mosaic Virus by RT PCR and SSCP |
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| Lei Juanli Chen Jiong Chen Jianping Zheng Tao Chen Ye. Identification of Chinese Fungal transmitted Filamentous Wheat Mosaic Virus by RT PCR and SSCP[J]. 中国病毒学(英文版), 1998, 0(1) |
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| Authors: | Lei Juanli Chen Jiong Chen Jianping Zheng Tao Chen Ye |
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| Affiliation: | Lei Juanli Chen Jiong Chen Jianping * Zheng Tao Chen Ye |
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| Abstract: | There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China. |
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| Keywords: | Fungal transmitted wheat mosaic virus RT PCR Single strand conformation polymorphisms Wheat yellow mosaic virus |
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