Production of (2S,3S)-2,3-butanediol and (3S)-acetoin from glucose using resting cells of Klebsiella pneumonia and Bacillus subtilis |
| |
Authors: | Liu Zhen Qin Jiayang Gao Chao Hua Dongliang Ma Cuiqing Li Lixiang Wang Yu Xu Ping |
| |
Institution: | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China. |
| |
Abstract: | Production of highly pure (2S,3S)-2,3-butanediol ((2S,3S)-2,3-BD) and (3S)-acetoin ((3S)-AC) in high concentrations is desirable but difficult to achieve. In the present study, glucose was first transformed to a mixture of (2S,3S)-2,3-BD and meso-2,3-BD by resting cells of Klebsiella pneumoniae CICC 10011, followed by biocatalytic resolution of the mixture by resting cells of Bacillus subtilis 168. meso-2,3-BD was transformed to (3S)-AC, leaving (2S,3S)-2,3-BD in the reaction medium. Using this approach, 12.5 g l(-1) (2S,3S)-2,3-BD and 56.7 g l(-1) (3S)-AC were produced. Stereoisomeric purity of (2S,3S)-2,3-BD and enantiomeric excess of (3S)-AC was 96.9 and 96.2%, respectively. |
| |
Keywords: | (2S 3S)-2 3-Butanediol (3S)-Acetoin Klebsiella pneumoniae Bacillus subtilis |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|