中国4个少数民族中SDF1多态性研究

研究与HIV 1感染相关的基质细胞衍生因子 (SDF1)等位基因突变频率和多态性在中国 4个少数民族的分布特征。应用PCR/ RFLP等方法检测回族 (5 7例 )、鄂伦春族 (71例 )、蒙古族 (30例 )及锡伯族 (2 6例 )共 184个个体中SDF1 -3’A基因突变频率。结果得出中国 4个民族中SDF1- 3’A基因的基因频率分别为 :蒙古族为 38 3%,锡伯族为 2 3 .1%,回族为 2 0 .2 %,鄂伦春族为 10 .6 %。中国 4个少数民族中SDF1- 3’A等位基因频率存在较大的差异 (χ2 =37 .82 6 ,P<0.01) , 提示这 4 个民族的遗传结构存在着一定的差异。 本研究 为评估中国不同民族对 HIV-1 的易感性及艾滋病的流行病学研究提供了基本数据。

STUDY ON POLYMORPHISM OF THE SDF1 ALLELE IN 4 ETHNIC GROUPS OF CHINA

To study the mutant frequency and polymorphism of stromal derived factor-1 (SDF-1) conferring resistance to determined HIV-1/AIDS in 4 ethnic groups in China. The studied population included 184 subjects,of which consisted the Hui (n=57),the Oroqen (n=71),the Mongolian (n=30),the Xibe (n=26).The genotype of the SDF1-3'A was detected by PCR/RFLP with the digestion of restriction endonuclease Msp I. The frequency of SDF1-3'A mutant allele was: 38.3% of the Mongolian, 23.1% of the Xibe, 20.2% of the Hui and 10.6% of the Oroqen. The difference of SDF1-3'A polymorphisms were found in 4 ethnic groups in China.