Ultrastructural localization of Lucifer Yellow and endocytosis in plant cells |
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Authors: | T P Owen Jr Kathryn A Platt-Aloia W W Thomson |
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Institution: | (1) Department of Botany and Plant Sciences, University of California, 92521 Riverside, CA, USA |
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Abstract: | Summary The fluorescent dye Lucifer Yellow CH (LYCH) was localized at the ultrastructural level with a precipitation method using barium chloride. Applying this technique, endocytosis of LYCH was examined in the nutrient absorptive trichomes of a carnivorous bromeliad. After a two hour incubation, the electron dense reaction product was localized in the membrane compartments of the endocytotic system. These structures included coated regions of the plasma membrane, coated and smooth vesicles, dictyosomes, partially coated reticulum, and smooth endoplasmic reticulum. This procedure demonstrates for the first time at the ultrastructural level endocytosis in whole plant cells, using a non-toxic compound.Abbreviations ER
endoplasmic reticulum
- BaCl2
barium chloride
- LYCH
Lucifer Yellow CH
- PCR
partially coated reticulum |
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Keywords: | Brocchinia reducta (Bromeliaceae) Coated vesicle Dictyosome Endocytosis Lucifer Yellow |
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