Cholecystokinin receptor mediated hydrolysis of inositol phospholipids in guinea pig gastric glands |
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Authors: | R S Chang V J Lotti T B Chen |
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Institution: | Department of Pharmacology Merck Sharp & Dohme Research Laboratories West Point, PA 19486, USA |
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Abstract: | CCK-octapeptide (CCK-8) (EC50 = 0.5 nM), in the presence of Li+, increased 3H-inositol phosphate (IP) accumulation in guinea pig gastric glands prelabeled with 3H-inositol. CCK-8 desulfate, human gastrin I and pentagastrin were much less potent than CCK-8. Antagonists of CCK receptors such as proglumide, dibutyryl-c-GMP and CBZ-Tyr (SO3H)-Met-Gly-Trp-Met-AspNH2 shifted the CCK dose response curve to the right. However, histamine (H1 and H2), cholinergic, substance P and alpha- and beta-adrenergic receptor antagonists had no effect on 3H-IP accumulation induced by CCK. The results suggest that CCK receptor activation in gastric glands leads to an enhanced breakdown of inositol phospholipids which may relate to calcium mobilization and pepsinogen secretion. |
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Keywords: | Send correspondence to: Raymond S L Chang Ph D Department of Pharmacology Merck Sharp & Dohme Research Laboratories West Point PA 19486 USA |
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