Selective neutrality of glucose-6-phosphate dehydrogenase allozymes in Escherichia coli |
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Authors: | Dykhuizen DE; de Framond J; Hartl DL |
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Institution: | Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110. |
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Abstract: | Six naturally occurring alleles representing four electromorphs of the
enzyme glucose-6-phosphate dehydrogenase were transferred by P1- mediated
transduction from natural isolates of Escherichia coli into the genetic
background of E. coli K12 and were studied in pairwise competition in
chemostats limited for glucose in order to estimate differences in growth
rate associated with the alleles. Although the level of resolution of such
experiments is a growth rate differential of approximately 0.002 h-1, no
significant differences among the strains were found. Studies of apparent
Km and Vmax in crude enzyme extracts of the strains also failed to reveal
any significant differences among the electromorphs. These results support
the view that the alleles are selectively neutral or nearly neutral under
these conditions.
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