Continuous culture of reuber hepatoma cells in serum-free,arginine-, glutamine-and tyrosine-deprived chemically defined medium |
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Authors: | Akira Niwa Katsuhiko Yamamoto Kenji Sorimachi Yosihiro Yasumura |
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Institution: | (1) Department of Microbiology, Dokkyo University School of Medicine, 321-02 Mibu, Tochigi, Japan |
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Abstract: | Summary The rat hepatoma cell line, H4-II-E, was grown serially over a I-year period and about 30 passages in arginine-, glutamine-,
and tyrosine-deprived and ornithine-supplemented Eagle's mininum essential medium with no supplements other than biotin. The
adapted cel line, R-Y121B, proliferates in the above mentioned medium with a doubling time of about 4 days and maintains hepatic
“marker” enzymes such as tyrosine aminotransferase, phenylalanine hydroxylase, and all the enzymes of the urea cycle.
This work was supported in part by Grant-in-Aid for Cancer Research 301050 and Science Research Grant 337013 from the Ministry
of Education, Science and Culture, Japan. |
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Keywords: | amino acid requirements differentiated hepatoma culture protein-free growth |
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