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Ostreolysin enhances fruiting initiation in the oyster mushroom (Pleurotus ostreatus) |
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| Authors: | Sabina Berne, Jure Pohleven, Iztok Vidic, Katja Rebolj, Franc Pohleven, Tom Turk, Peter Ma ek, Anton Sonnenberg,Kristina Sep i&#x |
| Affiliation: | aMedical centre for molecular biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia bDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, Slovenia cDepartment of Biotechnology, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia dDepartment of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, Rožna dolina, Cesta VIII/34, 1000 Ljubljana, Slovenia eDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia fDepartment of Plant Breeding, Wageningen University and Research Centre, P.O. Box 16, 6700 AA Wageningen, The Netherlands |
| Abstract: | Fruiting initiation in mushrooms can be triggered by a variety of environmental and biochemical stimuli, including substances of natural or synthetic origin. In this work ostreolysin, a cytolytic protein specifically expressed during the formation of primordia and fruit bodies of Pleurotus ostreatus, was applied to nutrient media inoculated with mycelium of P. ostreatus, and its effects on mycelial growth and fructification of the mushroom studied. The addition of ostreolysin slightly inhibited the growth of mycelium, but strongly induced the formation of primordia, which appeared 10 d earlier than in control plates supplemented with bovine serum albumin or with the dissolving buffer alone. Moreover, ostreolysin stimulated the subsequent development of primordia into fruit bodies. However, direct involvement of this protein in the sporulation of the mushroom is unlikely, as it was also detected in large amounts in the non-sporulating strain of P. ostreatus. |
| Keywords: | Fruiting initiation Ostreolysin Pleurotus ostreatus |
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