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Large fragment deletion using a CRISPR/Cas9 system in Saccharomyces cerevisiae
Authors:Huanhuan Hao  Xiaofei WangHaiyan Jia  Miao YuXiaoyu Zhang  Hui TangLiping Zhang
Affiliation:Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Key Discipline of Biological Engineering of Hebei Province, College of Life Sciences, Hebei University, Baoding, 071002, China
Abstract:Large chromosomal modifications have been performed in natural and laboratory evolution studies and hold tremendous potential for use in foundational research, medicine, and biotechnology applications. Recently, the type II bacterial Clustered Regularly Interspaced Short Palindromic Repeat and CRISPR-associated (CRISPR/Cas9) system has emerged as a powerful tool for genome editing in various organisms. In this study, we applied the CRISPR/Cas9 system to preform large fragment deletions in Saccharomyces cerevisiae and compared the performance activity to that of a traditional method that uses the Latour system. Here we report in S. Cerevisiae the CRIPR/Cas9 system has been used to delete fragments exceeding 30 kb. The use of the CRISPR/Cas9 system for generating chromosomal segment excision showed some potential advantages over the Latour system. All the results indicated that CRISPR/Cas9 system was a rapid, efficient, low-cost, and versatile method for genome editing and that it can be applied in further studies in the fields of biology, agriculture, and medicine.
Keywords:Large-fragments deletion   Latour system   CRISPR/Cas9   Comparation   Saccharomyces cerevisiae
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