转基因烟草中Bt毒蛋白基因的表达行为 |
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引用本文: | 石春林,朱祯,徐鸿林,肖桂芳,冯平章.转基因烟草中Bt毒蛋白基因的表达行为[J].Journal of Integrative Plant Biology,2000,42(3):269-273. |
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作者姓名: | 石春林 朱祯 徐鸿林 肖桂芳 冯平章 |
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Expression Behavior of Bt Toxin Gene in Transgenic Tobaccos |
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Authors: | SHI Chun-Lin ZHU Zhen XU Hong-Lin XIAO Gui-Fang FENG Ping-Zhang |
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Abstract: | Bt toxin genes were the insecticidal genes most widely used in genetic engineering of pest resistant plant, were of important significance to study their expression behavior in transgenic plants. In this work, a plant expression vector, pBinMoBc, was constructed. It contained the Cry IA(c) gene under control of chimeric OM promoter and the Ω factor. The vector was transferred into tobacco (Nicotiana tabacum L.) plant via Agrobacterium-mediated transformation. ELISA assay showed that the expression levels of the Cry IA(c) gene in transgenic tobacco plants were significantly higher than that in wild-type tobacco plants. The highest could be up to 0.255% of total soluble proteins; the expression level of CryIA(c) gene in transgenic tobacco plant was changeable during the development stages of tobacco plant. Bioassay showed that pBinMoBc transgenic tobacco plants had more notable insecticidal activity than the wild-type tobacco plants. The above results indicated that pBinMoBc was an effective pest-resistent plant expression vector. This study would be very helpful in screening transgenic cotton with high resistance to cotton bollworm (Heliothis armigeva Hubner). |
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Keywords: | transgenic plant Bt toxin gene effective promoter ELISA assay bioassay |
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