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Proteolytic degradation of heterologous proteins expressed inAspergillus niger
Authors:David B. Archer  Donald A. MacKenzie  David J. Jeenes  Ian N. Roberts
Affiliation:(1) AFRC Institute of Food Research, Norwich Research Park, Colney, NR4 7UA Norwich
Abstract:
Summary Proteolytic degradation of heterologous proteins expressed in the filamentous fungusAspergillus niger reduces the yield of authentic target protein. The activities ofA. niger proteases are differentiated by their effects on two proteins expressed and secreted fromA. niger: hen egg-white lysozyme and porcine pancreatic phospholipase A2.
Keywords:
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