Electrophysiological study of the action mechanism of somatoliberin (GH-RH) on hypophyseal GH3 tumor cells] |
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| Authors: | M Fahmi L Bresson L Dufy-Barbe P Sartor A Taupignon B Dufy |
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| Institution: | Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II. |
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| Abstract: | We have investigated the electrical response of patched GH3 cells to Growth-Hormone Releasing-Hormone (GH-RH). GH-RH (100 nM) enhanced firing frequency of action potentials. This is accompanied by membrane depolarization (5-10 mV) and conductance increase. Voltage clamp studies reveal that GH-RH potentiates calcium inward currents and a calcium-dependent chloride current; transient outward current is diminished. These changes in membrane conductance account for the cytosolic free calcium rise shown by Indo-1 fluorescence measurements. |
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