不同取卵时间对兔ICSI胚胎体外发育的影响

目的探讨不同取卵时间对兔ICSI胚胎体外发育的影响。方法采用Piezo操作系统对实验兔进行辅助体外受精。结果hCG注射后14、16、18h取卵，ICSI后的受精率分别为82.2％、75.9％和0.0％，对受精卵进行体外发育培养，桑椹胚的发育率分别为72.9％、70.0％、0.0％，囊胚的发育率分别为62.2％、53.3％、0.0％。14h和16h之间受精率、桑椹胚率、囊胚率差异不显著（P〉0.05），但是14h采卵比16h要好；18h和14h、16h之间差异显著（P〈0.05）。结论不同取卵时间影响实验兔的ICSI体外受精率及胚胎的体外发育率，hCG注射后14h取卵最有利于兔ICSI胚胎的发育。

Effects of Time of Oocyte Collection after Superovulation on in vitro Development of Intracytoplasmic Sperm Injection Rabbit Embryos

Objective This paper described the use of piezo-driven micropipette for intracytoplasmic sperm injection （ICSI） of rabbit eggs and discussed the effects of time of oocyte collection after superovulation on in vitro development of intracytoplasmic sperm injection rabbit embryos. Results Oocytes were collected at 14 h, 16 h, 18 h after hCG administration. The ICSI fertilization rate were 82.2% , 75.9% and 0.0%, respectively. The fertilized oocytes were further cultured in vitro. The morulae rates were 72.9% , 70.0% and 0.0%, and the rates of blastocyst were 62.2%, 53.3% and 0.0%, respectively. The rate of fertilization, morulae rate and the rate of blastocyst were not significantly different （ P 〉 0.05） between 14 h and 16 h groups, but were significantly different （ P 〈 0.05 ） between 14 h, 16 h and 18 h groups, but oocytes collected at 14 h after hCG administration were better than that of 16 h after hCG treatment. Conclusion Oocytes collected at different time after hCG injection has different effect on the fertilization rate of oocytes and development rate of intracytoplasmic sperm injection rabbit embryos. Oocytes collected at 14 h after hCG injection are most beneficial to the development of intracytoplasmic sperm injection rabbit embryos.