A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation,purification and biochemical characterization |
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Authors: | Xiaoyan Suo Xiuling Lu Tao Hu Guanghui Ma Zhiguo Su |
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Institution: | (1) National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, Beijing, 100190, People’s Republic of China;(2) Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China |
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Abstract: | A solid-phase adsorption method was developed to circumvent the disadvantage of the conventional liquid-phase PEGylation,
i.e. the heterogeneity of the PEGylated products. The model proteins, human serum albumin (HSA) and staphylokinase (SAK),
were adsorbed on the ion exchange chromatography media, followed by PEGylation with succinimidyl carbonate (SC)-mPEG5K and
salt elution. Since PEGylation with SC-PEG5K alters the positive charge of the proteins, Q-Sepharose Big Beads and DEAE Sepharose
Fast Flow were used for adsorption of HSA and SAK, respectively. Size exclusion chromatography and SDS-PAGE studies demonstrated
that solid-phase PEGylation of proteins generate monoPEGylated proteins with the yield of 35–47%. Circular dichroism and intrinsic
fluorescence studies showed that solid-phase PEGylation led to little conformational change of the proteins. Solid-phase PEGylation
resulted in 35% loss in the biological activity of SAK, which is lower than the liquid-phase PEGylation (70%). |
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Keywords: | Human serum albumin PEGylation solid-phase Staphylokinase |
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