Huperzine A--human serum albumin association: chromatographic and thermodynamic approach |
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Authors: | Darrouzain François André Claire Ismaili Lhassane Matoga Myriam Guillaume Yves Claude |
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Institution: | Equipe des Sciences Séparatives et Biopharmaceutiques (2SB) EA-482, Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besan?on Cedex, France. |
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Abstract: | The synthesis of six new huperzine analogues was reported. Each product presents an amidification of the free amine on huperzine A. The synthesis strategy of these new huperzine A derivatives is based on a condensation with an acyl anhydride. The binding on HSA of two molecule series (huperzine and benzodiazepine, respectively) was investigated with high performance liquid affinity chromatography (HPLAC) using an HSA column. A thermodynamic approach showed that binding huperzine A on HSA involved hydrophobic and Van der Waals interactions. A comparative thermodynamic study with benzodiazepine molecules was carried out to determine the potential binding site of huperzine derivatives on HSA. |
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