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| 一株鹅源H5N2亚型高致病性禽流感病毒的分离鉴定及生物学特性分析 | |
| 引用本文： | 刘春国,刘 明,张 云,刘大飞,潘蔚绮,孙恩成,杜金玲,李洪涛.一株鹅源H5N2亚型高致病性禽流感病毒的分离鉴定及生物学特性分析[J].生物化学与生物物理进展,2009,36(1):65-71. |
| 作者姓名： | 刘春国 刘 明 张 云 刘大飞 潘蔚绮 孙恩成 杜金玲 李洪涛 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医牛物技术国家霞点实验室,农业部动物流感中心及国家禽流感参考实验室,哈尔滨,150001 2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,禽传染病实验室,哈尔滨,150001 3. 中国科学院广州生物医药与健康研究院,广州,510663 4. 黑龙江八一农垦大学动物科技学院,大庆,163319 5. 中国农业科学院哈尔滨兽医研究所,兽医牛物技术国家霞点实验室,农业部动物流感中心及国家禽流感参考实验室,哈尔滨,150001;东北农业大学动物医学院,哈尔滨,150030 |
| 摘 要： | 分离到一株鹅源 H5N2亚型高致病性禽流感病毒，SPF鸡静脉接种致病指数为2.99，但鸭子对该病毒不敏感．病毒感染小鼠后不致病，但能够在肺内有效复制，表明其具有感染哺乳动物的潜在风险．血凝素(hemagglutinin, HA)蛋白裂解位点上插入有多个连续的碱性氨基酸(-RRRKKR-)，从分子上证实这是一株高致病性禽流感病毒．核酸序列比较分析表明，分离的流感病毒HA基因与A/chicken/Hubei/489/2004 (H5N1)同源率达到99.4%，神经氨酸酶(neuraminidase, NA)基因与A/chicken/Jilin/53/01(H9N2)同源率达到99.8%；氨基酸水平上，HA与2004年分离到的A/chicken/Hubei/489/2004(H5N1)、A/swan/Guangxi/307/2004(H5N1)、A/wildduck/Guangdong/314/ 2004(H5N1)和A/chicken/Henan/210/2004(H5N1)同源率均为99.3%，NA 与A/chicken/Jilin/53/01(H9N2)同源率为99.6%．进化树分析结果表明，该流感病毒分离株可能是由H5N1和H9N2两个亚型病毒重排而来． |
| 关 键 词： | 鹅 禽流感病毒 H5N2亚型 致病性 序列分析 |
| 收稿时间： | 5/1/2008 12:00:00 AM |
| 修稿时间： | 9/9/2008 12:00:00 AM |
Identification and Molecular Analysis of H5N2 Subtype Highly Pathogenic Avian Influenza Virus Isolated From Goose |
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| LIU Chun-Guo,LIU Ming,ZHANG Yun,LIU Da-Fei,PAN Wei-Qi,SUN En-Cheng,DU Jin-Ling and LI Hong-Tao.Identification and Molecular Analysis of H5N2 Subtype Highly Pathogenic Avian Influenza Virus Isolated From Goose[J].Progress In Biochemistry and Biophysics,2009,36(1):65-71. | |
| Authors: | LIU Chun-Guo LIU Ming ZHANG Yun LIU Da-Fei PAN Wei-Qi SUN En-Cheng DU Jin-Ling and LI Hong-Tao |
| Institution: | Animal Influenza Center of The Ministry of Agriculture and The National Avian Influenza Reference Laboratory, National Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;Animal Influenza Center of The Ministry of Agriculture and The National Avian Influenza Reference Laboratory, National Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;Avian Infectious Disease Division, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;Animal Influenza Center of The Ministry of Agriculture and The National Avian Influenza Reference Laboratory, National Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;Guangzhou Institute of Biomedicine and Health, The Chinese Academy of Sciences Guangzhou 510663, China;College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China;Animal Influenza Center of The Ministry of Agriculture and The National Avian Influenza Reference Laboratory, National Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;Animal Influenza Center of The Ministry of Agriculture and The National Avian Influenza Reference Laboratory, National Key Laboratory of Veterinary Biotechnology , Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001, China;College of Animal Medicine, Northeast Agricultural University, Harbin 150003, China |
| Abstract: | A H5N2 subtype avian influenza virus isolated from goose belongs to highly pathogenic avian influenza virus, and the intravenous pathogenicity indexes (IVPI) =2.99. But ducks are not sensitive to this isolated influenza virus. The virus can infect mouse but only replicates in lung and has no pathogenicity. HA and NA gene of this isolated strain share 99.4% and 99.8% nucleotide sequence identity to the HA gene of A/chicken/Hubei/ 489/2004 (H5N1) and the NA gene of A/chicken/Jilin/53/01(H9N2), and share 99.3% and 99.6% amino acid sequence identity to the HA protein of A/chicken/Hubei/489/2004(H5N1), A/swan/Guangxi/307/2004(H5N1), A/wild duck/ Guangdong/314/2004(H5N1), A/chicken/Henan/210/2004(H5N1) and the NA protein of A/chicken/ Jilin/53/01(H9N2). There are several continuous basic amino acids (-RRRKKR-) at the cleavage site of HA protein. Phylogenetic trees analysis of HA and NA gene suggests that the isolated influenza virus probably originated from the reassortment of H5N1 and H9N2 subtype influenza virus. |
| Keywords: | goose avian influenza virus subtype H5N2 pathogenicity sequence analysis |
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