Changes in photosynthetic activity,pigment composition,electrolyte leakage,lipid peroxidation,and antioxidant enzymes during ex vitro establishment of micropropagated <Emphasis Type="Italic">Rauvolfia tetraphylla</Emphasis> plantlets |
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Authors: | M Faisal M Anis |
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Institution: | (1) Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India |
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Abstract: | The effects of photosynthetic photon flux density (PPFD) on antioxidant metabolism and photosynthetic properties in leaves
during ex vitro establishment of micropropagated Rauvolfia tetraphylla plantlets were investigated. In vitro-propagated plantlets were acclimatized at either 50 (Low-light = LL) or 300 (High-light = HL)
μmol m−2s−1 photosynthetic PPFD for 4 weeks under controlled conditions. Increases in chlorophyll (Chl) a, b and carotenoid levels were observed in plantlets acclimatized at both light intensities. At transplantation, micropropagated
plantlets were not photosynthetically active, but the net photosynthetic rate increased in newly formed leaves over time during
acclimatization. The observed differences in pigment contents and photosynthetic rates suggested adaptation of plantlets from
heterotrophic to autotrophic mode of nutrition during acclimatization. Changes in activities of antioxidant enzymes were also
observed during acclimatization. Superoxide dismutase activity increased in plantlets acclimatized at HL intensities. Likewise,
changes in activity of catalase and ascorbate peroxidase were also detected. These observed changes reflected the ability
of plants in developing an antioxidant enzymatic defense system aiding in survival against oxidative stress and in reducing
release of free radicals. |
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