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Genomic and phenomic differentiation of Rhodococcus equi and related strains |
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| Authors: | Elizabeth J. McMinn Grace Alderson Hilary I. Dodson Michael Goodfellow Alan C. Ward |
| Affiliation: | (1) Department of Biomedical Sciences, University of Bradford, Bradford, BD7 1DP, UK;(2) Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK;(3) Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK |
| Abstract: | 16S rDNA sequence and pyrolysis mass spectrometric analyses were carried out on representatives of Rhodococcus equi and marker strains of genera that encompass mycolic acid containing actinomycetes. The R. equi strains formed a monophyletic clade within the evolutionary radiation occupied by members of the genera Nocardia and Rhodococcus. The 16S rDNA sequence data also showed R. equi to be an heterogeneous taxon. This heterogeneity was underscored by the pyrolysis mass spectrometric data. These observations are in line with those of previous studies where similar profiles of relatedness were found between pyrolysis mass spectral data and the results of DNA:DNA pairing and numerical phenetic studies. |
| Keywords: | Pyrolysis mass spectrometry Rhodococcus equi 16S rDNA sequencing |
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