The Influence of Specific Phospholipids on the Interaction of Hexokinase with the Outer Mitochondrial Membrane |
| |
Authors: | F Moller John E Wilson |
| |
Institution: | Department of Biochemistry and The Neuroscience Program, Michigan State University, East Lansing, Michigan, U.S.A. |
| |
Abstract: | Repeated washing of a brain mitochondrial fraction results in a progressive decrease in the proportion of mitochondrially bound hexokinase that can be solubilized during a subsequent incubation with glucose-6-phosphate (glucose-6-P). Phospholipids removed during the washing procedure can be added back to washed mitochondria, resulting in enhancement of the solubilization by glucose-6-P. Column and thin-layer chromatographic methods have been used to isolate and identify active phospholipids. Additional studies were performed with purified lipids obtained commercially. Both lysophospholipids and acidic phospholipids were active in enhancing solubilization of hexokinase by glucose-6-P. Phospho-inositides, particularly diphosphoinositide, were quite effective, raising the possibility that the actively metabolized phosphoinositides may be involved in regulation of hexokinase binding in vivo. |
| |
Keywords: | Hexokinase Mitochondria Phosphatidylinositol response Phosphoinositides Phospholipids Lysophospholipids |
本文献已被 PubMed 等数据库收录! |
|