Anchoring of bovine chromosomes 4, 6, 7, 10, and 14 linkage group telomeric ends via FISH analysis of lambda clones |
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| Authors: | Timothy P L Smith Nestor Lopez-Corrales Michael D Grosz Craig W Beattie Steven M Kappes |
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| Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, Nebraska 68933-0166, USA, US;(2) U.S. Department of Agriculture, ARS, Fort Keogh LARRL, Miles City, Montana 59301, USA, US |
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| Abstract: | We report the placement of 34 new microsatellite (ms) markers, isolated from a lambda phage genomic clone library, on the
bovine genetic map by linkage to published markers. Five of these markers lie at or near the ends of linkage groups and are
used to establish chromosomal coverage and orientation. Fluorescence in situ hybridization (FISH) analysis demonstrates that
the linkage groups on the U.S. Meat Animal Research Center (MARC) map extend to the telomeric region of Chromosomes (Chrs)
7 and 10. Linkage groups on Chrs 4, 6, and 14 appear to be less inclusive.
Received: 23 September 1996 / Accepted: 28 December 1996 |
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