Effects of capsule hardness on germination frequency of encapsulated somatic embryos of carrot |
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| Authors: | J. Shigeta T. Mori K. Toda H. Ohtake |
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| Affiliation: | (1) Biotechnology & Environmental Engineering Department, Ishikawajima-Harima Heavy Industries Co., Ltd., 235 Yokohama, Japan;(2) Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Summary Somatic embryos of carrot were encapsulated in calcium alginate beads to provide artificial carrot seeds. Alginate capsules with a hardness of 0.2 to 0.5 kg/cm2 were found to be suitable for germination of encapsulated somatic embryos. The germination frequencies were more than 95%, when grown aseptically on polyester fiber supports loaded with hormone-free Murashige-Skoog medium. |
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