| 大鼠肥厚心肌细胞钙电流及 Na~ /Ca~(2 )交换电流的研究(英文) |
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| 作者姓名： | Lu JY Wu DM Wu BW Chai WX Kang CS Li TL |
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| 作者单位： | 山西医科大学生理教研室!太原,030001,山西医科大学生理教研室!太原,030001,山西医科大学生理教研室!太原,030001,山西医科大学第一医院!太原,030001,山西医科大学第一医院!太原,030001,山西医科大学第一医院!太原,030001 |
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| 摘 要： | 本文观察了心肌肥厚对大鼠心肌细胞Na ／Ca2 交换电流的影响。我们采用Goldblatt两肾一夹方法诱发大鼠心肌肥厚,应用全细胞膜片钳技术记录电流。结果表明：肥厚心肌细胞的Ni2 -敏感Na ／Ca2 交换电流密度大于正常细胞。在钳制电压为＋50mV时,正常细胞的外向交换电流密度为1．53±0．31pA／pF,而肥厚细胞则为2．62±0．53pA／pF（P＜0．01）;钳制电压为-100mV时,正常细胞的内向交换电流密度为0．42±0．14pA／pF,肥厚细胞达1．12±0．33pA／pF（P＜0．001）。这些结果提示,肥厚心肌细胞的Na ／Ca2 交换电流发生了改变,其意义有待进一步探讨。
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| 关 键 词： | 大鼠 心肌肥厚 膜片钳 Na~ ／Ca~(2 )交换电流 |
Na+/Ca2+ exchange current in myocytes isolated from rat hypertrophied heart |
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| Lu JY,Wu DM,Wu BW,Chai WX,Kang CS,Li TL.Na+/Ca2+ exchange current in myocytes isolated from rat hypertrophied heart[J].Acta Physiologica Sinica,1999,51(5):588-592. |
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| Authors: | Lu J Y Wu D M Wu B W Chai W X Kang C S Li T L |
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| Institution: | Department of Physiology, Shanxi Medical University, Taiyuan 030001. |
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| Abstract: | Effects of myocardial hypertrophy on the Na+/Ca2+ exchange current in isolated rat cardiac myocytes were investigated, using whole cell patch techniques. Goldblatt 2-kidney, one-clip technique was used to induce cardiomyocyte hypertrophy. The results demonstrated that the Ni(2+)-sensitive Na+/Ca2+ exchange current density was larger in hypertrophied cells than that in normal cells. At a holding potential of mV, the outward current densities were 1.53 +/- 0.31 pA/pF in normal cells and 2.62 +/- 0.53 pA/pF in hypertrophied cells (P < 0.01). At a holding potential of -100 mV, the inward current densities were 0.42 +/- 0.14 pA/pF in normal cells and 1.12 +/- 0.33 pA/pF in hypertrophied cells (P < 0.001). The results suggest that during myocardial hypertrophy the Na+/Ca2+ exchange current is significantly increased. |
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| Keywords: | rat myocardial hypertrophy patch-clamp techniques Na~ /Ca~(2 ) exchange current |
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