Discrimination of <Emphasis Type="Italic">Tricholoma</Emphasis> species by species-specific ITS primers |
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| Authors: | Kensuke Kikuchi Norihisa Matsushita Kazuo Suzuki |
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| Institution: | (1) Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) Laboratory of Forest Microbiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan |
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| Abstract: | The ITS region of ectomycorrhizal fungi was analyzed, and species-specific PCR primers were designed for 8 ectomycorrhizal
Tricholoma species. Although a high degree of intraspecific homology was observed, interspecific variation was sufficient to design
species-specific primers based on sequence of the ITS region. PCR amplification with the specific primers generated fragments
of the expected sizes from DNA extracted from the strains of each species but gave no amplified products from the strains
of the other 16 species in eight genera. These results suggest that sequence of the ITS region is appropriate to be used for
species-level identification of ectomycorrhizal fungi. |
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| Keywords: | Ectomycorrhizal fungi Internal transcribed spacer (ITS) region Species-specific primer Tricholoma species |
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